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The aim of this study was to compare the intervals of time between adjacent zero crossings (ZCI), an alternative frequency-temporal parameter, with the root-mean-square (RMS) value and the median frequency (Fmed) from the surface EMG (sEMG) signal in muscle fatigue analysis. Twenty right-handed volunteers performed isometric contractions of right biceps brachii muscle while sEMG signals were collected from it at three different and arbitrary load levels until fatigue. The mean ZCI presented a significant correlation with Fmed but not with RMS value and it also presented lower coefficients of variation than others. The results pointed that mean ZCI properties can contribute more than Fmed and RMS value on the interpretation of the muscle function under fatigue conditions.