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An investigation of an alkaline thermostable amylase secreted by Bacillus amyloliquefaciens MTCC-610 strain was carried out in order to test its compatibility and wash performance as a detergent additive. The optimum pH and temperature for amylase activity was found to be 8.0 and 50°C. The enzyme was quite stable for 1 h at 400C while at 500C, 50% of the original activity was lost, respectively. The compatibility of Bacillus amyloliquefaciens amylase with certain commercial detergents was shown to be good as the enzyme retained 96.4%, 94% and 86% of its activity after 1hr of incubation at 40°C in the presence of the detergent brands Surf Excel Blue, Surf Excel and Fena Bar respectively. The wash performance of detergents (7 mg/ml) prepared with enzyme amylase was studied on cotton, terrycloth and chiffon fabric samples stained with potato curry sample. Maximum stain removal was observed for chiffon samples where supplementation of the enzyme to the detergent Surf excel blue enhanced the cleansing ability of the detergent followed by cotton and terrycloth. We report here the compatibility and wash performance of alkaline amylase from Bacillus amyloliquefaciens MTCC-610.
Date of Conference: 10-12 Sept. 2010