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In graphics, gravure printing is the preferred method for printing high quality, fine dimension graphics using high-speed roll-to-roll or sheet fed presses. Gravure printing typically employs flexible and compressible substrates such as various papers and polymer films. In electronics, glass substrates are a common, if not preferred, substrate in many applications, particularly displays and photovoltaics. In combining printing with glass substrates, challenges exist in adapting contact-based printing methods such as gravure to the mechanical properties of the more rigid substrates. In this work, sheet-fed gravure printing has been successfully used to print silver-based conductive inks on glass substrates. Various features were designed and printed to evaluate conductive layers in terms of their printability and electrical performance. The independent variables include gravure cell dimensions, trace orientation with respect to printing direction and ink type. Results from this work provide an insight into the science of gravure printing on glass by correlating the independent variables to printed feature quality and electrical performance.