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The practical teaching is a key to train students in practical ability and innovative consciousness. But, evaluation standards of practice teaching are still confusion. By analyzing the characteristics of practice teaching, we put forward the evaluation method of practical teaching on the core of Knowledge Transfer. In accordance with this method, the examination paper has been designed. Through the analysis of the test paper, some important characteristics of practical teaching can be revealed. It is not only able to correctly evaluate the students ability to master and apply knowledge, it also has some guidance for teachers how to improve practical teaching.