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Conventional coagulation is not an effective treatment method to remove natural organic matter (NOM) and ammonia nitrogen (NH3-N) in water at low temperature. For this type of water, enhanced coagulation is currently proposed as one of the feasible treatment methods and is implemented by coagulant optimization based on raw water characteristics and the coagulant aids. NOM and NH3-N removal from Huaihe river water have been studied through an enhanced coagulation process with polyaluminum chloride (PAC), polyacrylamide (PAM) and diatomite (100-120μm) in this work. The effectiveness of the tested coagulants was evaluated by measurements of turbidity, CODMn UV254 and NH3-N. The diatomite, PAC and PAM combination of enhanced coagulation was presented, and results showed that: when 100 mg/L diatomite, 30 mg/L PAC and 0.6 mg/L PAM were added, the removal efficiency of turbidity, NH3-N, CODMn and UV254 were respectively 98.5%, 35.9%, 48.8% and 49.4%, and the removal rates were increased by 3.7%, 14.2%, 14.3% and 19.1% compared with single dosing PAC (30 mg/L). The experiment results can act as a reference for the optimization of the process of coagulation.