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The problem to be solved is in situ remediation of ground water contaminated by nitrobenzene through simulation of underground surroundings and integrated application of PRB and chemical and biological reactive barriers. Firstly, deoxidize the nitrobenzene to aniline by the deoxidization of the zero valent iron in the chemical reactive barrier; secondly, decompose the residual nitrobenzene by degrading bacteria in the biological reactive barrier. The results show the rates of deoxidization and decomposition are 99.44% and 99.91% respectively. Integrated use of both methods mentioned above can provide a theoretical basis for the in-situ remediation of ground water contaminated by high concentration nitrobenzene.