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A color CCD (charge-coupled device) camera is used as a chemical detector to quantify fluorescence emission intensities from two kinds of optofluidic device platforms. The fluorophores responsive to oxygen are immobilized in photo-patternable hydrogel matrices. The first device is a hydrogel sensor array formed within a commercial silicone elastomer imaging chamber. The second one involves a double-chamber fluidic device composed of a permeable membrane layer and dry film photoresist layers. The results suggest that digital color imagers, in combination with a simple color separation method, can serve as the high quality chemical detectors for fluidic biosensors and biological culture platforms.
Date of Conference: 1-2 July 2010