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Nowadays, information security is always the most important topic. With the help of the USB Key and fingerprint, people have developed kinds of authentication solutions to protect the confidential information against unauthorized access. After some research, we found that the above solutions have two serious problems. One is that the sensitive information is not encrypted; the other one is that the environment is insecure. In this paper, we developed a new high-level security portable system to solve these problems. The system is a combination of hardware and software. On the one hand, based on the USB Key and fingerprint scanner, we integrated a plug-and-play USB2.0 device without driver installation. On the other hand, by rebuilding the syslinux boot loader and customizing the Puppy Linux and doing some special works on the USB flash partition, we have developed and integrated a fingerprint-based authentication security portable custom-built software environment inside the USB device. With the help of the system, the users can work at a movable security environment freely, as well as the high-level protection of confidential information.