By Topic

Amplification circuit and microelectrode array for HL-1 Cardiomyocyte action potential measurement

Sign In

Cookies must be enabled to login.After enabling cookies , please use refresh or reload or ctrl+f5 on the browser for the login options.

Formats Non-Member Member
$31 $13
Learn how you can qualify for the best price for this item!
Become an IEEE Member or Subscribe to
IEEE Xplore for exclusive pricing!
close button

puzzle piece

IEEE membership options for an individual and IEEE Xplore subscriptions for an organization offer the most affordable access to essential journal articles, conference papers, standards, eBooks, and eLearning courses.

Learn more about:

IEEE membership

IEEE Xplore subscriptions

3 Author(s)
Jianan Song ; Electr. Eng., Arizona State Univ., Tempe, AZ, USA ; Welch, D. ; Christen, J.B.

We have designed and tested two amplification circuits for measuring HL-1 cardiac action potentials, single-ended and differential. We describe the improvements in performance resulting from the differential configuration. These improvements are especially important considering the cell culture's aqueous environment that experiences DC drift. In addition we have designed and fabricated a 4×4 gold microelectrode array and cultured HL-1 cells on the electrodes. The array is connected to our circuit through a custom made Pogo pin jig to allow for easy measurements from the electrodes while cells are being cultured. This setup allowed us to obtain initial results for modeling the transfer function of the cell culture as a passive device network. Testing has confirmed the circuits are capable of filtering low frequency signals including DC drift and frequencies above 5 kHz. The amplification was achieved through two gain stages with a total gain of 60 dB for the single-ended and 46 dB for the differential circuits.

Published in:

Circuits and Systems (ISCAS), Proceedings of 2010 IEEE International Symposium on

Date of Conference:

May 30 2010-June 2 2010