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For the drinking water treatment, a methodology for isolation of the bacterium capable of removing 2-methylisoborneol (2-MIB) from water was proposed. The procedure of the method was as follows: the sample was gained from the activated carbon whose surface was covered with a mature microbiological membrane, and then continually treated with the mineral salt liquid culture in which 2-MIB was the only carbon source. Finally, four strains were isolated by plate streaks, which were characterized as Flavobacterium spp. and Pseudomonas spp. based on the physiology biochemistry experiment and the data of 16S rRNA analysis.