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A cold plasma micro-jet (PMJ) has been shown very effective in inactivating numerous kinds of bacteria. However, when treating eukaryocytes, such as yeasts or human cells lines, the effects of a cold plasma on them are more complicated than simple inactivation. This may be due to the complex pathways within eukaryotic cells in response to different kind of stress. Here we used the budding yeast S. cerevisiae, a well-studied model, to investigate the eukaryotic response pathways to a treatment by a cold plasma. Different single deletion mutants of S. cerevisiae were exposed to an atmospheric-pressure, cold plasma microjet and their respective viability after treatment was measured.