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In this paper, we report the development of methods for the stable localization of DNA origami as bionanostructures for potential molecular optoelectronic sensor systems. The microcontact printing approach has been adapted to provide a rapid method for the production of self-assembled monolayer nanopatterning of aminopropyltriethoxysilane (APTES) on mica substrates. 1.2 μm blazed gratings were used as molds in the production of soft polydimethylsiloxane stamps. This stamp has been used to prepare fine-line patterns of APTES on flat mica surfaces. A solution-phase method was used to coat the bare mica between these APTES lines with dichlorodimethylsilane, which suppresses nonspecific binding of DNA origami nanostructures. Imaging studies confirm the addressing of rectangular DNA origami structures to the APTES patterns on mica.
Date of Publication: Sept. 2010