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Effect of light irradiation and immobilization of carbon nanotubes on kinetic parameters corresponding to Michaelis — Menten equation, for NADH sensors based on phenothiazine graphite modified electrodes

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2 Author(s)
M. -L. Ungure├žan ; Technical University of Cluj-Napoca, Chemical Department, 400641 Cluj-Napoca ; D. -M. Gligor

The presence of light irradiation and immobilization of carbon nanotubes on graphite electrodes modified with polyphenothiazine formaldehyde (PPF) and bis-phenothiazin-3-yl methane (BPhM) leads to an increasing of sensitivity for NADH detection (calculated as Imax/KM ratio). The paper presents the analogical modelling and numerical simulation for fitting the Michaelis-Menten equation to experimental data by a regression analysis. The program determines the theoretical kinetic parameters: KM and Imax and they are compared with the values of the same parameters experimentally determined.

Published in:

Automation Quality and Testing Robotics (AQTR), 2010 IEEE International Conference on  (Volume:3 )

Date of Conference:

28-30 May 2010