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The Cloning and Analysis of a Partial Lipase Gene Sequence of Staphylococcus hominis GIMT1.079: Partial Lipase Gene Sequencing of S. hominis

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7 Author(s)
Qiao Na-na ; Coll. of Biotechnol., Tianjin Univ. of Sci. & Technol., Tianjin, China ; Gao Qiang ; Zhao Qin ; Wang De-pei
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Lipase activity is common in staphylococci. Based on the blast of various known staphylococcal lipase DNA sequences, the degenerate primers were derived to amplify a 782-bp consensus lipase gene sequence of Staphylococcus hominis GIMT1.079, which encoded a deduced polypeptide of 260 amino acid (aa) residues. The alignment of aa sequence revealed that this 260-aa partial lipase of S. hominis shared high homology with those conserved parts of other 11 deduced staphylococcal lipases, in the range of the lowest 43.0% of S. epidermidis and the highest 63.5% of S. saprophyticus. Two aa residues, Ser39 and Asp230, were preliminarily confirmed in the putative catalytic triad as well as the 'P-loop' motif (-[AG]-x4-G-K-[ST]-) in this deduced 260-aa partial S. hominis lipase sequence.

Published in:

Bioinformatics and Biomedical Engineering (iCBBE), 2010 4th International Conference on

Date of Conference:

18-20 June 2010