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Isolation and Identification of Two Degrading Strains in Atrazine Contaminated Soil

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5 Author(s)
Ying Zhang ; Sch. of Resource & Environ., Northeast Agric. Univ., Harbin, China ; Chunyan Li ; Zhuo Diao ; Shuyan Ma
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Two strains named W7 and W16 were isolated by liquid enrichment culture from the corn field where atrazine has been chronically applied. Observed by gram stain and SEM, W7 belonged to gram-positive bacteria and the single cell was short bacilliform; W16 belonged to gram-negative bacteria and the single cell was spherical. Through physiological and identification and 16SrDNA sequence analysis, two strains were identified as Rhizobium sp. and Rhodobacter sphaeroides sp. respectively. W7 and W16 could degrade atratine effectively, and the biodegradation rate reaching up to 99 % after 72 h cultivation in suitable conditions.

Published in:

Bioinformatics and Biomedical Engineering (iCBBE), 2010 4th International Conference on

Date of Conference:

18-20 June 2010