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Denitrification is one of the main objectives of water pollution control. By using heterotrophic nitrification- aerobic denitrifying bacterial strains H1 to deal with domestic sewage, the removal rate of TN and NH4+-N can reach 66.04% and 62.11% respectively. In order to achieve solid-liquid separation and recycling of strains, H1 immobilized by polyvinyl alcohol- sodium alginate, Fe3O4 nanoparticles, bacterial cellulose was used to deal with domestic sewage. The sewage treatment effects of the immobilized methods are all superior to free bacterial strains. Besides, H1 immobilized by two adsorption modes of bacterial cellulose achieve 77.22 % and 69.81% of effluent total nitrogen removal rates, respectively. In view of environment-friendly property of bacterial cellulose as immobilization carrier, H1 immobilized by bacterial cellulose has a great prospect in the field of sewage treatment.