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JProNet is a Java-based network tool for systematic analysis of structure-based topological properties of proteins. It incorporates new developments in network theory such as the scale-free and the small-world properties. JProNet can generate rich information of the residue packing topology of protein structure networks. JProNet is a platform independent, interactive and user-friendly tool. Users are empowered with the flexibility to input their customized protein structure-based network and define the weight of the edges in the network prior to the analysis.