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In this paper, the motivation is to integrated chemical and biomedical separation, analysis and assay techniques into a PCB technology-based system with LED light source, smart CMOS vision sensor and lab-on-a-chip device. One high sensitivity low noise pinned-photodiode smart CMOS vision sensor is designed and fabricated in this paper. The measurement shows that the maximum sensitivity is about 0.95V/sec.lux. The chip operates in the normal condition of 30fps@54M clock and its noise level is low to below the 0.8mV level due to the pinned pixel technology, noise reduction circuit and design optimization. Based on the smart CMOS vision sensor, one PCB technology-based optical analytical system has been built up for lab-on-a-chip. This proposed printed circuit board (PCB) technology system has small size, low power consumption, low cost and real hand-held device and can avoid possible human errors with automation and low labor.