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Integrated Photoacoustic and Fluorescence Confocal Microscopy

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5 Author(s)
Yu Wang ; Dept. of Biomed. Eng., Washington Univ. in St. Louis, St. Louis, MO, USA ; Maslov, K. ; Chulhong Kim ; Song Hu
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We have developed a dual-modality imaging system by integrating optical-resolution photoacoustic microscopy and fluorescence confocal microscopy to provide optical absorption and fluorescence contrasts simultaneously. By sharing the same laser source and objective lens, intrinsically registered photoacoustic and fluorescence images are acquired in a single scan. The micrometer resolution allows imaging of both blood and lymphatic vessels down to the capillary level. Simultaneous photoacoustic angiography and fluorescence lymphangiography were demonstrated, presenting more information to study tumor angiogenesis, vasculature, and microenvironments in vivo.

Published in:

Biomedical Engineering, IEEE Transactions on  (Volume:57 ,  Issue: 10 )

Date of Publication:

Oct. 2010

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