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The problem of reconstructing three-dimensional objects buried in layered media is addressed in this paper. A numerical approach which is based on an Estimation of Distribution Algorithm(EDA) is proposed. The reconstruction is obtained from the iterative inversion method that minimizes the cost function by using EDA, where the cost function is defined as the squared magnitude of the difference between the measured fields and the calculated fields from the assumed profile of the object. Numerical result shows these methods are capable of reconstructing an arbitrary 3D inhomogeneous object buried in a multilayered medium.