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This paper deals with the development of a low cost self-sensing Piezeoresistive Microcantilever Biosensor to detect human stress using salivary alpha amylase activity. The salivary alpha amylase enzyme will immobilize on to the Piezeoresistive Microcantilever biosensor to detect activity during the enzyme responses. When the Microcantilever beam deflects it caused the stress change within the microcantilever beam and applied strain to the piezoresistor material thereby causing the resistance change and the Piezoresistive Microcantilever biosensor integrated with transducer components coverts the biochemical signal into measurable signal. The enzyme concentration signal is converted to a voltage by the transducer. A novel Piezeoresistive Microcantilever biosensing method and its application is described. The device was designed specifically that it enables the small resistivity change due to the enzymatic reaction to be measured.