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Specific Protein Detection in Multiprotein Coexisting Environment by Using LSPR Biosensor

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6 Author(s)
Shuhong Li ; State Key Lab. of Opt. Technol. for Microfabrication, Chinese Acad. of Sci., Chengdu, China ; Shaoyun Yin ; Yueyun Jiang ; Changcheng Yin
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Specific protein detection in multiprotein coexisting environment by localized surface plasmon resonance (LSPR) biosensor is studied. After the surfaces of self-assembly fabricated triangular Ag nanoparticles on the chip are activated, a specific biological molecule layer with δ-toxic protein identifying function is linked to the chip. Based on the LSPR spectrometer measurement system in our laboratory, δ-toxic protein in sonicated Bacillus thuringiensis cell fractions (which consists of many kinds of proteins) is detected successfully by measuring the extinction spectrum of the chip.

Published in:
Nanotechnology, IEEE Transactions on  (Volume:9 ,  Issue: 5 )

Date of Publication: Sept. 2010

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