Specific protein detection in multiprotein coexisting environment by localized surface plasmon resonance (LSPR) biosensor is studied. After the surfaces of self-assembly fabricated triangular Ag nanoparticles on the chip are activated, a specific biological molecule layer with δ-toxic protein identifying function is linked to the chip. Based on the LSPR spectrometer measurement system in our laboratory, δ-toxic protein in sonicated Bacillus thuringiensis cell fractions (which consists of many kinds of proteins) is detected successfully by measuring the extinction spectrum of the chip.
Published in:
Nanotechnology, IEEE Transactions on
(Volume:9
,
Issue:
5
)
Date of Publication: Sept. 2010