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In this letter, we report on the inscription of a fourth-order fiber Bragg grating made line-by-line in the optical fiber using a femtosecond laser. Strong Bragg resonance (~17 dB) and low insertion loss (~0.5 dB) were obtained with only 2000 periods. Measured refractive index change of these inscribed lines reaches up to 7 × 10-3. The grating was fully characterized and the low insertion loss together with low polarization-dependent loss were realized compared to gratings made by the point-by-point method. The high temperature annealing experiment shows the grating can survive up to at least 800°C.