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This paper describes a flexible microplasma jet device using a Tygon® S-54-HL tube as a biocompatible tube and its potential in developing cancer therapies. The optical and physical properties of the plasma jets and preliminary apoptosis data of cultured murine tumor cells and nontumor fibroblast cells treated with these plasma jets are presented. Microplasma jets were observed to induce apoptosis in cultured murine cells in a dose-dependent manner. The murine melanoma tumor cells were more sensitive to plasma treatment than fibroblast cells. These features allow the direct and precise application of this microplasma jet device to tumor cells.