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J2EE-based Web applications are becoming increasingly ubiquitous and with their increasing adoption, the performance is the attention focus and the most important factor of evaluating the system by users. In this paper, we present a systematic solution for performance testing and optimization of J2EE-based Web applications. The solution helps to identify and eliminate bottlenecks in the application design and ensures that systems are designed to meet their quality of service requirements. This paper firstly analyses the architecture of J2EE-based Web applications and performance testing principle, and then improves the JMeter testing framework for meeting the more concurrent users. Lastly, performance testing for J2EE-based Web applications is done; it finds performance bottlenecks and puts forward optimum measures, and compares the performance with the former one.