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Thin-film transistors (TFTs) were fabricated using an indium oxide (In2O3) thin film as the n-channel active layer by RF sputtering at room temperature. The TFTs showed a thickness-dependent performance in the range of 48-8 nm, which is ascribed to the total carrier number in the active layer. Optimum device performance at 8-nm-thick In2O3 TFTs had a field-effect mobility of 15.3 cm2 · V-1 · s-1, a threshold voltage of 3.1 V, an ON-OFF current ratio of 2.2 × 108, a subthreshold gate voltage swing of 0.25 V · decade-1, and, most importantly, a normally OFF characteristic. These results suggest that sputter-deposited In2O3 is a promising candidate for high-performance TFTs for transparent and flexible electronics.