By Topic

A microfluidic system integrated with optical detection devices for automatic detection of C-reactive protein

Sign In

Cookies must be enabled to login.After enabling cookies , please use refresh or reload or ctrl+f5 on the browser for the login options.

Formats Non-Member Member
$31 $13
Learn how you can qualify for the best price for this item!
Become an IEEE Member or Subscribe to
IEEE Xplore for exclusive pricing!
close button

puzzle piece

IEEE membership options for an individual and IEEE Xplore subscriptions for an organization offer the most affordable access to essential journal articles, conference papers, standards, eBooks, and eLearning courses.

Learn more about:

IEEE membership

IEEE Xplore subscriptions

6 Author(s)
Wen-Bin Lee ; Dept. of Eng. Sci., Nat. Cheng Kung Univ., Tainan, Taiwan ; Yin-He Chen ; Hsin-I Lin ; Chao-June Huang
more authors

C-reactive protein (CRP) is commonly known as a clinical marker, which has been identified as an independent predictor for cardiovascular diseases such as heart attack and stroke. In this study, a new microfluidic system for automatic measurement of CRP is reported. The entire process including sample pre-treatment, incubation between target CRP and anti-CRP antibody, developing process and detection can be automatically performed on a single chip. In addition, chemiluminescent signal was measured by using a home-made luminometer to quantify the concentration of CRP. When compared to previous works, not only can the new chip perform the entire process by integrating a new micro-injector, but a new CRP-specific DNA aptamer was also used for CRP measurement. Experimental data showed that the developed system can automate the entire process within 30 minutes with a detection limit of 0.0125 mgL-1, which was superior to previous works.

Published in:

Micro Electro Mechanical Systems (MEMS), 2010 IEEE 23rd International Conference on

Date of Conference:

24-28 Jan. 2010