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Micro-CEC chip with gradient hydrophobic stationary phase (GHSP) provided by mwcnts nanocolumns-applied to protein analysis in MALDI-TOF-MS

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5 Author(s)
Hsuan-Hsuan Lin ; Eng. & Syst. Sci. Dept., Nat. Tsing Hua Univ., Hsinchu, Taiwan ; Ren-Guei Wu ; Hsiao-Yu Chen ; Chung-Shi Yang
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In this research, we present a chip based capillary electrochromatography (CEC) carrying out a highly efficient separation of biomolecules through gradient hydrophobic stationary phase (GHSP) provided by multiwalled carbon nanotubes (MWCNTs) nanocolumns. 4-azidoaniline was employed for the surface modification of the MWCNTs to form a gradient stationary phase by a photochemical reaction through a gradient light filter. This device was used for separating proteins (20 ¿M FITC-BSA and FITC-Cytochrome C) with different hydrophobic characteristics, and MALDI-TOF-MS detection was employed to verify the separation efficiency in this MWCNTs nanocolumn. The best separation resolution of the FITC-BSA column efficiency was 2.5 × 104 (plats/m).

Published in:

Micro Electro Mechanical Systems (MEMS), 2010 IEEE 23rd International Conference on

Date of Conference:

24-28 Jan. 2010