By Topic

Smaller structures taking the lead - analysis and simulation of structure size influences on binding kinetics down to the single molecule level

Sign In

Cookies must be enabled to login.After enabling cookies , please use refresh or reload or ctrl+f5 on the browser for the login options.

Formats Non-Member Member
$33 $13
Learn how you can qualify for the best price for this item!
Become an IEEE Member or Subscribe to
IEEE Xplore for exclusive pricing!
close button

puzzle piece

IEEE membership options for an individual and IEEE Xplore subscriptions for an organization offer the most affordable access to essential journal articles, conference papers, standards, eBooks, and eLearning courses.

Learn more about:

IEEE membership

IEEE Xplore subscriptions

5 Author(s)
Phillip Kuhn ; HSG-IMIT, Wilhelm-Schickard-Straße 10, 78052 Villingen-Schwenningen, Germany ; Nils Paust ; Roland Zengerle ; Felix von Stetten
more authors

This paper describes a method for the quantitative detection of biochemical binding events onto microstructured and functional surfaces on a truly single molecular level. The classic Streptavidinbiotin system is used to provide the last detection step and the binding is visualized via gold-nanoparticles in a SEM. We showed that this allows a spatial resolution down to the nanometer scale. It also allowed us to proof the spot size dependence of binding kinetics according to the theorem of Ekins in one single experiment. The method allows to analyze any binding event on a planar surface and is enabling to measure surface densities of functional groups like the amount of BSA molecules on a blocked glass surface.

Published in:

Micro Electro Mechanical Systems (MEMS), 2010 IEEE 23rd International Conference on

Date of Conference:

24-28 Jan. 2010