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Label-free detection of oligonucleotide microarrays by oblique-incidence reflectivity difference method

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10 Author(s)
Wang, Xu ; Beijing National Laboratory for Condensed Matter Physics, Institute of Physics, Chinese Academy of Sciences, Beijing 100190, China ; Yuan, Kun ; Lu, Heng ; Wen, Juan
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Hybridizations between labeled or label-free targets and corresponding 21-base oligonucleotide probes, concentrations of which range from 0.39 to 50 μM, are detected by oblique-incidence reflectivity difference (OI-RD) method and fluorescence detection. The experimental results demonstrate that the OI-RD method can be utilized to not only distinguish whether the hybridization of oligonucleotides happened but also directly tell the different concentrations of the labeled and unlabeled oligonucleotides on the microarrays. The analysis with a classical three-layer model suggests that single-strand DNA tends to lie on epoxy-functionalized glass slide while the double-strand DNA prefers to have a tilted angle with respect to the slide in our experimental situation. The label-free detection of hybridization of oligonucleotides declares that OI-RD is a promising method for label-free and high-throughput detection of the biological microarrays.

Published in:

Journal of Applied Physics  (Volume:107 ,  Issue: 6 )

Date of Publication:

Mar 2010

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