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Zirconia based nucleic acid sensor for Mycobacterium tuberculosis detection

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4 Author(s)
Das, Maumita ; Department of Science and Technology Centre on Biomolecular Electronics, National Physical Laboratory, Dr. K. S. Krishnan Marg, New Delhi 110012, India ; Sumana, Gajjala ; Nagarajan, R. ; Malhotra, B.D.

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Nanostructured zirconium oxide (ZrO2) film (particle size∼35 nm), electrochemically deposited onto gold(Au) surface, has been used to immobilize 21-mer oligonucleotide probe (ssDNA) specific to Mycobacterium tuberculosis by utilizing affinity between oxygen atom of phosphoric group and zirconium to fabricate DNA biosensor. This DNA-ZrO2/Au bioelectrode, characterized using x-ray diffraction, Fourier transform infrared spectroscopy, cyclic voltammetry, and scanning electron microscopy techniques, can be used for early and rapid diagnosis of M. tuberculosis with detection limit of 0.065 ngL within 60s.

Published in:

Applied Physics Letters  (Volume:96 ,  Issue: 13 )