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With the continuing development of biotechnology and nanotechnology, a miniaturized and integrated device that is capable of controlling and measuring a small droplet has become desirable. Using a surface acoustic wave (SAW), a droplet is manipulated. If the sensor is fabricated onto the SAW propagating surface, a novel sensor and actuator integrated system, namely digital microfluidic system, is realized. The integrated device is called a Â¿micro-laboratory.Â¿ In this paper, to actualize a disposable micro-laboratory, three-layer structure of sensor plate/ matching layer/ piezoelectric substrate is examined. Droplet manipulation, mixing and measurement are succeeded using the proposed novel micro-laboratory.