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This paper presents an optimization methodology by using a genetic algorithm (GA) to obtain the parameters of a soil that can be represented in a multilayer structure. The method uses a curve of experimental apparent resistivity obtained from measurements made in the soil. This experimental curve is compared with another curve of apparent resistivity of the soil, theoretical, produced by the GA. The theoretical curve is based on Sunde's Algorithm and is exactly the inverse process used for the horizontal stratification of the soil in multilayers. With both curves in hand, the error produced in the process of soil stratification can be estimated. From the estimated errors, the parameters are optimized. The main difference of this method, comparing the already developed methods is just that, besides optimizing the resistivities ρi and the thicknesses hi of each soil layer, the proposed method also optimizes the layers quantity, seeking the best soil stratification. The obtained results of this paper are compared with other soil stratification methods.