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Nucleotide-mediated size fractionation of gold nanoparticles in aqueous solution

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3 Author(s)
Wenting Zhao ; Bioeng. Grad. Program, Hong Kong Univ. of Sci. & Technol., Kowloon, China ; Li Lin ; I-Ming Hsing

In this work, we present a novel approach for the size fractionation of gold nanoparticles (Au-nps), which combines the nucleotide-mediated stabilization mechanism for Au-nps to the size-dependent colloidal stability of nanoparticles. Under nucleotide-coating, Au-nps undergo reversible salt-induced aggregation in aqueous solutions, while the critical salt concentration (CSC) for the transition of Au-nps aggregation increase as the nanoparticle size getting smaller. Successful fractionation of both binary and ternary mixtures composed of Au-nps in 10 nm, 20 nm and 40 nm were demonstrated and the final purity of each fraction is larger than 90%. The whole process can be accomplished within one hour due to the rapid stabilization of Au-nps with nucleotide-mediation.

Published in:

Nanoelectronics Conference (INEC), 2010 3rd International

Date of Conference:

3-8 Jan. 2010