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Interest in protein sensors using guided-mode resonance (GMR) filters is rapidly growing as the demand for sensitive and reliable protein sensors increases in clinical diagnostic applications and pharmaceutical research. GMR filter-based protein sensors are capable of high sensitivity in the detection of molecular interactions, by measuring the movement of sharp reflectance peaks. We designed a GMR filter by computer simulation, and created a prototype by UV nanoimprinting, using a highly durable metallic nano stamp, which was fabricated by electroforming process with a polymeric master pattern. We also demonstrated the use of this GMR filter as a protein sensor by measuring the peak wavelength value (PWV) and the PWV shift.