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In this study, we investigated that flagellated bacteria Serratia marcescens had been utilized as actuators for SU-8 microstructures through the bovine serum albumin-selective patterning. Firstly, we analyzed the adherence of the bacteria to SU-8 microstructure through the selective patterning using 5% BSA. As a result, the number of attached bacteria in 5 surfaces coated-microstructure with BSA was increased 180% compare with non-treated microstructure. Moreover, the number of attached-bacteria to in all surfaces coated microstructure with BSA was decreased 67% less than the adhesion of the bacteria in non-treated microstructure. Secondly, when moving distance and velocity of 5 surfaces coated-microstructure with BSA was increased 224% compared with those of the non-treated microstructure. The results revealed that the bacteria patterning to specific site using 5% BSA makes an important role of the increment of the microstructure motility actuated by bacteria.