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Eco-water is defined as a transformation of precipitation, which is deposited by vegetation layer, humiliated vegetation layer and soil layer. It plays an important role in the water-cycle system. As potential factors on Eco-water and Eco-water layer are different from one season to another, multi-temporal and multi-type remote sensing data, measured spectrum and the routine observation were applied to construct the indices for Eco-water and its inversion model. The four Eco-water indices, including Vegetation Canopy Interception Content, Vegetation Water Content Index, Soil Moisture Index and Eco-water Storage Index, were calculated. The results show that the RS information model can reflect the real soil moisture. The dissertation brings forward the Eco-water Remote Sensing quantitative study based on vegetation layer. The vegetation-based calculation model for Eco-water with quantitative remote rensing technology, which has been identified in the dissertation, possesses significant science affect and practical value; and it can not only advance the methods of Eco-environment study, but also promote the research on water-resources transformation and water-cycle, also enlarge the domains of remote sensing applications.