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To observe the influence of hyperthermia under different temperature on the growth, apoptosis of Human Lung Cancer cell of A549, H1299 to investigate the possible mechanism of apoptosis in lung cancer cell caused by p53 with different activity. Human lung cancer cell of A549, H1299 was heated under 40Â°C 42Â°C 44Â°C separately for 2 hours each in the water bath case. After the hyperthermia under different temperature, the inhibition ability of A549 cells and H1299 cells were determined by MTT, the apoptosis and cell cycle of A549 cells and H1299 cells were analyzed by FACS, and RT-PCR was adopted to detect the expression of the apoptosis related gene of p53 and caspase-3.
Date of Conference: 13-14 Dec. 2009