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A leakage current microsensor for detection of interaction between an electrolyte-entrapping liposome and protein

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7 Author(s)
Noda, M. ; Grad. Sch. of Sci. & Technol., Kyoto Inst. of Technol., Kyoto, Japan ; Asai, T. ; Yamashita, Kaoru ; Shimanouchi, T.
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We have newly made a specific biomolecule microsensor measuring leakage current and impedance with minute liposome solution that interacts with biomolecules such as protein. The microsensor was fabricated with a Si surface-bulk-micromachining process. K4[Fe(CN)6] solution was entrapped inside of liposome of 1,2-dipalmitoyl-sn-glycero-3-phosphocholline (DPPC) and Carbonic Anhydrase from Bovine (CAB) was used as added protein for the interaction. A clear leakage in nA order was observed for the CAB, indicating the microsensor detects the CAB through the liposome-protein interaction. The detectivity increased by about 7 times and the volume of solution was decreased by about 10,000 from the previous result.

Published in:

Sensors, 2009 IEEE

Date of Conference:

25-28 Oct. 2009