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Fiber optic bio-sniffer (biochemical gas sensor) using UV-LED light for monitoring ethanol vapor with high sensitivity & selectivitiy

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7 Author(s)
Kudo, H. ; Inst. of Biomater. & Bioeng., Tokyo Med. & Dental Univ., Tokyo, Japan ; Miyajima, K. ; Takahashi, D. ; Arakawa, T.
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A fiber optic bio-sniffer (biochemical gas sensor) for alcohol gas monitoring with high sensitivity and high selectivity was fabricated and tested. The bio-sniffer is a gas sensor that uses molecular recognition of enzyme to improve selectivity. Usually, enzyme loses activity in the gas phase. Applying a flow-cell with a gas-intake window to the sensing probe, enzyme immobilized at the sensing region was kept in the sufficient wet condition to maintain activity. The bio-sniffer measures ethanol (EtOH) vapor by measuring fluorescence of nicotinamide adenine dinucleotide (NADH), which is produced by enzymatic reaction at the flow-cell. In order to construct a simplified system suitable for on-site applications, a high-intensity ultraviolet light emitting diode (UV-LED) was utilized as an excitation light. Owing to low power consumption comparing with previous light sources, the bio-sniffer was considered to be suitable for laptop applications such as on-site monitoring. According to the characterization, the bio-sniffer for was useful for continuous alcohol monitoring and showed high selectivity. The calibration range was 0.30-300 ppm which is suitable for evaluation of capacity to metabolize alcohol.

Published in:

Sensors, 2009 IEEE

Date of Conference:

25-28 Oct. 2009