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Avian influenza-DNA hybridization detection using wavelength interrogation-based surface plasmon resonance biosensor

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7 Author(s)
Shin Ae Kim ; Sch. of Electr. Eng. & Comput. Sci., Seoul Nat. Univ., Seoul, South Korea ; Sung June Kim ; Sang Hun Lee ; Tai Hyun Park
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A wavelength interrogation-based surface plasmon resonance (SPR) biosensor has been investigated for a detection of avian influenza DNA (AI-DNA). The hybridization reaction between AI-DNA and the immobilized oligonucleotide probe on a gold surface was monitored. The results showed that SPR biosensor can provide a linear detection suitable for quantification of AI-DNA hybridization as well as a large dynamic range. The experimental results were consistent well with numerical calculations using rigorous-coupled-wave analysis (RCWA). This study demonstrates the potential of using wavelength-scanning type SPR biosensor in a simple and real-time detection of AI virus.

Published in:

Sensors, 2009 IEEE

Date of Conference:

25-28 Oct. 2009

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