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Micro cell analysis device using cellular photothermal effect and thermal sensor

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5 Author(s)
Bong Seop Kwak ; Dept. of Mech. Eng., Yonsei Univ., Seoul, South Korea ; Beom Seok Kim ; Suk-Heung Song ; Hyung Hee Cho
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This article presents a new micro-device for analysis of a laser-induced cellular photothermal effect using a microfabricated platinum resistance temperature detector (platinum RTD). The thermometer was fabricated with platinum thin layer using a microelectromechanical system (MEMS) technique and the microchannel was made of polydimethylsiloxane (PDMS) using a standard soft-lithography. Platinum RTD has 2 ¿m of the electrode width with the serpentine shaped platinum element and the electric resistance at 0°C is 247.88 ¿. To measure erythrocyte-specific heat variations, 1 to 300 mW power tunable diode pumped solid state (DPSS) laser module was used with 532 nm wavelength. Temperature variations of blood cells after laser-irradiation were measured using the platinum RTD. For manipulating the cells, a microfluidic channel and hydrodynamic focusing were used.

Published in:

Sensors, 2009 IEEE

Date of Conference:

25-28 Oct. 2009