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The application of enterprise mobile information system has become an irreversible tendency and trend in the world. But the present mobile system has problems such as bad security, not unified data transmission standard and so on. In view of these problems, and the characteristic of mobile network environment, the paper proposes a solution to the security of enterprise mobile information system. In this plan, technology of Single Sign-On is used to carry on the authentication of the mobile subscriber. The public key infrastructure based on PKI data encryption and the digital signature technology is employed to solve the problem of security of data transmission, integrity and the identifiability and non-repudiation between two parties and so on. The XML technology is used to solve the data transmission standard problem. Finally, this paper puts forward J2ME and Java EE 5.0 platform for the validation of the program.