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Aimed at the shortcoming of the traditional theory and method in the analysis of lake water environment evolution mechanism, a method based on multi-agent was introduced. The lake water environment system is regarded as complex system, which is composed of many relatively self-governed agents. These agents behaviors were modeled and simulated to analyze the macroevolution process of lake water environment system. The feasibility of multi-agent method was discussed in emphases, and a lake water environment multi-agent model was proposed. Finally, a simulation experiment to analyze the evolutionary game behavior of the agents in our model was implemented, to prove the effectiveness of this model. The results of simulation verified the rationality of this method.