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The purpose of this paper is to study the decision-making problem of knowledge sharing for supply chain enterprises. First, knowledge sharing process is modeled as a Stackelberg game with one manufacturer and one supplier. According to the equilibrium outcome of this game, the equilibrium values of total input for knowledge-sharing, the member's participation rate and the total expected profit are determined. On this basis, conditions for the formation of knowledge-sharing cooperation are explored from the perspective of profit distribution. Furthermore, with the introduction of cooperative effect factor and degree of knowledge difference factor, impact of relation characteristics on the knowledge sharing decision is investigated.