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In this paper, we present the evaluation of human's emotion by using brain waves. As an honest characteristic of fluctuating of human's emotion, EEG electroencephalography (EEG) has been studied by many researchers these years. P300 as an important endogenous component of event-related potential (ERP), affects people's concentration of attention. In general EEG researches, comfortableness was always concerned, while achievement and nervousness was rarely reported. In this paper, we try to measure and compare P300 by achievement stimulus and nervousness stimulus. The experiment is a game called Â¿Finding DifferenceÂ¿, which focuses on the visual irritation. By Â¿Signal AveragingÂ¿, we abstract the ERP, and studied the appearance of P300 (P: positive, 300: latency of 300 ms), and estimate the concentration stimulated by achievement and nervousness. As the result, we found that in the case of nervousness stimulus, P300 appeared more apparently than that in the case of achievement stimulus.