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In the past decade, microfabrication of miniature fluidic devices has attracted considerable interest and made substantial impact. One of the most promising applications is for biomedical applications, especially for fast diagnosis. A microfluidic system, which integrates sample pretreatment, transportation, reaction, separation, and detection on a small chip, can be realized by combining functional microfluidic components. When compared with their large-scale counterparts, the miniature system may provide advantages including compactness, lower cost, less sample/reagent consumption, automation, and higher sensitivity. In this talk, three microfluidic chips will be reviewed, including (1) an integrated microfluidic chip for fast diagnosis of infectious diseases; (2) an integrated microfluidic chip capable of performing fast detection of generic diseases; (3) an integrated microfluidic system for ELISA detection. It may provide a promising platform for clinical applications.