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To meet the demand of high data rate transmissions for multimedia wireless communications, orthogonal frequency division multiplexing (OFDM) systems in conjunction with multiple-input multiple-output (MIMO) signal processing have been considered one of the central techniques in advanced wireless communications. In the paper, two semiblind channel estimation algorithms are proposed for the uplink multiuser OFDM systems with insufficient guard interval, in contrast to sufficient guard interval assumed in most of the prior works. A zero-padding OFDM system, which zero-pads rather than cyclicly prefixing each block, is considered in this paper. By utilizing the relation between the linear prediction error filters (LPEFs) of the received signal with multiple prediction orders and the transmitted data sequence, the first proposed algorithm, namely the multistage LP (MLP) based algorithm, can estimate the MIMO channel coefficients, with only a single pilot OFDM block used. To reduce the sensitivity of the proposed algorithms to the channel order overestimation, it is proposed to implement the LPEFs with a QR-decomposition based approach. This QRdecomposition based approach alternatively computes the LPEFs without direct inversion of the received signal correlation matrix, thus exhibiting robustness against channel order overestimation. Some simulation results are presented to demonstrate the effectiveness and robustness of the proposed algorithms.