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A prototype microwave imaging system for imaging 3-D targets in layered media is developed to validate the capability of microwave imaging with experimental data and with 3-D nonlinear inverse scattering algorithms. In this experimental prototype, the transmitting and receiving antennas are placed in a rectangular tub containing a fluid. Two plastic slabs are placed in parallel in the fluid to form a five-layer medium. The microwave scattering data are acquired by mechanically scanning a single transmitting antenna and a single receiving antenna, thus avoiding the mutual coupling that occurs when an array is used. The collected 3-D experimental data in the fluid are processed by full 3-D nonlinear inverse scattering algorithms to unravel the complicated multiple scattering effects and produce 3-D digital images of the dielectric constant and conductivity of the imaging domain. The image reconstruction is focused on the position and dimensions of the unknown scatterers. Different dielectric and metallic objects have been imaged effectively at 1.64 GHz.