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Robust adaptive multiuser detection schemes are developed for direct-sequence code-division multiple-access (DS-CDMA) multipath frequency-selective fading channels. Multiple access interference (MAI) and intersymbol interference (ISI) are presented in identical format in the expanded signal subspace, which provides convenience for symbol-by-symbol multiuser detection. The proposed multiuse detectors are designed in the expanded signal subspace, and subspace estimation and Kalman filtering algorithms are developed for their adaptive implementation. It is demonstrated by simulation that these adaptive detectors are robust against subspace estimation error and can effectively suppress both MAI and ISI and converge to the optimum SINR.